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Sample GSM170895 Query DataSets for GSM170895
Status Public on Feb 24, 2007
Title Resistant Replicate 5
Sample type RNA
 
Source name cell line
Organism Homo sapiens
Characteristics KBM-7/B5 cloe 1803
Treatment protocol grown in tissue culture medium
Extracted molecule total RNA
Extraction protocol Qiagen column extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U95A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the HP GeneArray Scanner .
Description See manuscript (Leukemia Research, 2007).
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Feb 22, 2007
Last update date Mar 16, 2009
Contact name Reinhold Munker
E-mail(s) [email protected]
Phone 318-675-8770
Fax 318-675-4969
Organization name LSU Health Sciences Center
Department Medicine
Lab Division of Hematology/ Oncology
Street address 1501 Kings Highway
City Shreveport
State/province LA
ZIP/Postal code 71130
Country USA
 
Platform ID GPL8300
Series (1)
GSE7114 Comparative analysis of a CML cell line resistant to cyclophosphamide using oligonucleotide arrays and response to TKI

Data table header descriptions
ID_REF Affymetrix Probe Set ID
VALUE GCOS-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
100_g_at 2021.1 A 0.175989
1000_at 2376 P 0.011447
1001_at 525.3 A 0.35869
1002_f_at 334.3 A 0.715285
1003_s_at 73.9 A 0.88284
1004_at 728.5 M 0.05447
1005_at 1332.8 A 0.138765
1006_at 83.5 A 0.47938
1007_s_at 1063.6 A 0.127645
1008_f_at 7182.4 P 0.000959
1009_at 42108.6 P 0.000266
101_at 811.9 A 0.107263
1010_at 70 A 0.824011
1011_s_at 733.1 P 0.00418
1012_at 96.7 A 0.901946
1013_at 1315.4 P 0.017001
1014_at 3804.5 P 0.004863
1015_s_at 327.1 A 0.824011
1016_s_at 54.1 A 0.960635
1017_at 388.4 A 0.204022

Total number of rows: 12625

Table truncated, full table size 323 Kbytes.




Supplementary file Size Download File type/resource
GSM170895.CEL.gz 1.3 Mb (ftp)(http) CEL

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