|
| Status |
Public on Dec 29, 2015 |
| Title |
Fetal Liver 2 |
| Sample type |
genomic |
| |
|
| Source name |
Fetal Liver
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
age: 20wk
|
| Treatment protocol |
Liver was flash frozen in liquid nitrogen at the time of harvest and stored at -80 until use
|
| Growth protocol |
Normal adult liver was obtained through the The Liver Tissue Cell Distribution System is funded by NIH Contract #HHSN276201200017C. Fetal liver was obtained from spontaneous stillbirths occuring between 20-22wk gestation
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using the Qiagen All prep kit according to the manufacturer's instructions.
|
| Label |
Cy3 and Cy5
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium 450k Human Methylation Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using Hiscan according to manufacturer's instructions
|
| Description |
Normal Fetal Liver
|
| Data processing |
GenomeStudio software
Unmethylated and methylated signal intensities
|
| |
|
| Submission date |
Jun 12, 2015 |
| Last update date |
Dec 30, 2015 |
| Contact name |
Jennifer Ann Sanders |
| E-mail(s) |
[email protected]
|
| Organization name |
Rhode Island Hospital
|
| Department |
Pediatric Endocrinology
|
| Street address |
593 Eddy Street
|
| City |
Providence |
| State/province |
RI |
| ZIP/Postal code |
02903 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE69852 |
Patterns of DNA methylation in human fetal and adult liver |
|
