|
| Status |
Public on Nov 02, 2007 |
| Title |
PCCl3_24h_1 |
| Sample type |
SAGE |
| Anchor |
NlaIII
|
| Tag Count |
21216 |
| Tag Length |
10 |
| |
|
| Source name |
PCCl3 thyroid follicular cell, 24h
|
| Organism |
Rattus norvegicus |
| Characteristics |
Rat thyroid cell
|
| Biomaterial provider |
James A. Fagin
|
| Growth protocol |
Cell were cultured in Ham's F12 medium supplemented with 5% calf serum and the four hormones: 1mU/ml bovine TSH, 10ug/ml insulin, 5ug/ml transferrin and 10nM hydrocortisone.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Polyadenylated RNA was isolated using magnetic beads provided by I-SAGE Kit (Invitrogen)
|
| |
|
| Description |
PCCl3 cells were cultured without any additional treatment
|
| |
|
| Submission date |
Mar 02, 2007 |
| Last update date |
Nov 19, 2008 |
| Contact name |
Edna Teruko Kimura |
| E-mail(s) |
[email protected]
|
| Phone |
55-11-30917304
|
| Fax |
55-11-30917402
|
| Organization name |
USP - University of Sao Paulo
|
| Department |
ICB - Cell and Developmental Biology
|
| Lab |
Thyroid Molecular Biology
|
| Street address |
Av Prof Lineu Prestes 1524 sala 414
|
| City |
Sao Paulo |
| State/province |
SP |
| ZIP/Postal code |
05508000 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL23 |
| Series (1) |
| GSE7174 |
Differential gene expression analysis of iodide treated rat thyroid follicular cell line PCCl3 |
|
