|
Status |
Public on Feb 24, 2010 |
Title |
hES_SA01_2 |
Sample type |
RNA |
|
|
Source name |
human embryonic stem (hES) cells line SA01, biological replicate 2
|
Organism |
Homo sapiens |
Characteristics |
human ES cells line from Cellartis, Sweden; commercial line derived from normal embryos Karyotype: X,Y
|
Growth protocol |
The hES cell lines were maintained on a feeder layer of mitomycin C-inactivated murine embryonic fibroblast (MEF) cells. The culture media (DMEM/F12 supllemented with 20% KRS and 4ng/ml bFGF) was changed daily and routine passages were performed by mechanical cutting of hES cells on a fresh feeder layer every 4-5 days.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from the cells using the RNeasy Mini kit (Qiagen) according to the manufacturer protocol
|
Label |
Biotin
|
Label protocol |
100 ng of total RNA was used to generate biotinylated cRNA using the GeneChip® Two-Cycle Target Labeling and Control Reagents from Affymetrix according to the manufactures protocol
|
|
|
Hybridization protocol |
Hybridisation, washing and staining procedures of the Affymetrix GeneChip® Human Genome U133 Plus 2.0 were carried out as described in the Affymetrix technical manual (see manufacturer's web site)
|
Scan protocol |
Scanning procedures of the Affymetrix GeneChip® Human Genome U133 Plus 2.0 were carried out as described in the Affymetrix technical manual (see manufacturer's web site)
|
Description |
gene expression data from undifferentiated hES cells line SA01
|
Data processing |
The statistical treatment of the expression data was achieved using the Array Assist 4.1 software (Stratagene). Normalization was performed using the GC-RMA algorithm.
|
|
|
Submission date |
Mar 02, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Genevieve PIETU |
E-mail(s) |
[email protected]
|
Phone |
33169471154
|
Fax |
33169471153
|
Organization name |
INSERM
|
Department |
U861
|
Lab |
ISTEM
|
Street address |
1 rue de l'Internationale
|
City |
Evry |
ZIP/Postal code |
91000 |
Country |
France |
|
|
Platform ID |
GPL570 |
Series (2) |
GSE7179 |
Comparison of gene expression data between wild-type and DM1-affected undifferentiated hES cells. |
GSE7214 |
Comparison of gene expression data between wild-type and DM1-affected cells |
|
Relations |
Reanalyzed by |
GSE119087 |