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Sample GSM172878 Query DataSets for GSM172878
Status Public on Feb 24, 2010
Title hES_SA01_2
Sample type RNA
 
Source name human embryonic stem (hES) cells line SA01, biological replicate 2
Organism Homo sapiens
Characteristics human ES cells line from Cellartis, Sweden; commercial line derived from normal embryos
Karyotype: X,Y
Growth protocol The hES cell lines were maintained on a feeder layer of mitomycin C-inactivated murine embryonic fibroblast (MEF) cells. The culture media (DMEM/F12 supllemented with 20% KRS and 4ng/ml bFGF) was changed daily and routine passages were performed by mechanical cutting of hES cells on a fresh feeder layer every 4-5 days.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from the cells using the RNeasy Mini kit (Qiagen) according to the manufacturer protocol
Label Biotin
Label protocol 100 ng of total RNA was used to generate biotinylated cRNA using the GeneChip® Two-Cycle Target Labeling and Control Reagents from Affymetrix according to the manufactures protocol
 
Hybridization protocol Hybridisation, washing and staining procedures of the Affymetrix GeneChip® Human Genome U133 Plus 2.0 were carried out as described in the Affymetrix technical manual (see manufacturer's web site)
Scan protocol Scanning procedures of the Affymetrix GeneChip® Human Genome U133 Plus 2.0 were carried out as described in the Affymetrix technical manual (see manufacturer's web site)
Description gene expression data from undifferentiated hES cells line SA01
Data processing The statistical treatment of the expression data was achieved using the Array Assist 4.1 software (Stratagene). Normalization was performed using the GC-RMA algorithm.
 
Submission date Mar 02, 2007
Last update date Aug 28, 2018
Contact name Genevieve PIETU
E-mail(s) [email protected]
Phone 33169471154
Fax 33169471153
Organization name INSERM
Department U861
Lab ISTEM
Street address 1 rue de l'Internationale
City Evry
ZIP/Postal code 91000
Country France
 
Platform ID GPL570
Series (2)
GSE7179 Comparison of gene expression data between wild-type and DM1-affected undifferentiated hES cells.
GSE7214 Comparison of gene expression data between wild-type and DM1-affected cells
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE GC-RMA value

Data table
ID_REF VALUE
1007_s_at 196.86856
1053_at 656.3624
117_at 7.4473386
121_at 18.842382
1255_g_at 523.8367
1294_at 5.993323
1316_at 17.926157
1320_at 5.992838
1405_i_at 9.721613
1431_at 6.342167
1438_at 4.9056177
1487_at 109.41707
1494_f_at 5.5179663
1552256_a_at 342.2587
1552257_a_at 789.8082
1552258_at 4.993242
1552261_at 6.682925
1552263_at 78.594536
1552264_a_at 70.46966
1552266_at 9.974727

Total number of rows: 54675

Table truncated, full table size 1087 Kbytes.




Supplementary file Size Download File type/resource
GSM172878.CEL.gz 7.9 Mb (ftp)(http) CEL

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