Age: 2 y, when sample taken Gender: M Race: Hispanic Illness: Pneumonia/Empyema Treatment: Ceftriaxone/Vancomycin Pathogen: S. pneumoniae
Extracted molecule
total RNA
Extraction protocol
RNeasy mini
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol
EukGE-WS2v5_450
Scan protocol
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 System.
Description
The subject was infected with S. pneumoniae.
Data processing
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
