|
| Status |
Public on Apr 21, 2007 |
| Title |
107T |
| Sample type |
RNA |
| |
|
| Source name |
107T Human colon tumor tissue sample
|
| Organism |
Homo sapiens |
| Characteristics |
tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolated from cell culture using RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized, washed and stained using the Affymetrix Fluidics Station 400 according to Affymetrix standard procedures
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
Gene expression data from human colon tumor tissue
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
|
| |
|
| Submission date |
Mar 06, 2007 |
| Last update date |
Apr 20, 2007 |
| Contact name |
Mark David Ayers |
| E-mail(s) |
[email protected]
|
| Phone |
609-818-3055
|
| Organization name |
Bristol-Myers Squibb
|
| Department |
Clinical Discovery Technologies
|
| Lab |
Oncology Biomarkers
|
| Street address |
311 Pennington-Rocky Hill Rd.
|
| City |
Pennington |
| State/province |
NJ |
| ZIP/Postal code |
08534 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE7208 |
Expression data from 59 human colon tumor tissue samples |
|
