|
| Status |
Public on Oct 31, 2007 |
| Title |
6 hr RSL1, biological rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
Cells exposed to RSL1 for 6 hr
|
| Organism |
Homo sapiens |
| Characteristics |
LNCaP prostate cancer cells, clone 7-11, 6 hr after induction of AIbZIP expression
|
| Treatment protocol |
Cells were incubated in medium containing vehicle alone (Control) or in medium containing RSL1 (500 nM) for 6 or 24 hr.
|
| Growth protocol |
RPMI 1640 supplemented with 10% fetal calf serum, 2 mM L-glutamine, 50 ug streptomycin/ml and 100 IU penicillin/ml. Cells were grown at 37 C in 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cells using Trizol Reagent, according to the manufacturer’s protocol (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 20 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C to GeneChip U133 Plus 2.0 Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000 G7.
|
| Description |
Gene expression data from clone 7-11 cells, 6 hr following addition of RSL1
|
| Data processing |
The data were analyzed with LimmaGUI software (Bioconductor). Data was normalized by RMA method.
|
| |
|
| Submission date |
Mar 08, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Ezequiel L Calvo |
| E-mail(s) |
[email protected]
|
| Organization name |
CRCHUL
|
| Department |
Molecular Endocrinilogy
|
| Lab |
Microarrays
|
| Street address |
2705 Boul. Laurier
|
| City |
Quebec |
| State/province |
Quebec |
| ZIP/Postal code |
G1V 4G2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE7223 |
Genes regulated by the processed form of AIbZIP/CREB3L4 in LNCaP prostate cancer cells |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
