|
| Status |
Public on Mar 12, 2007 |
| Title |
0621m_hd |
| Sample type |
genomic |
| |
|
| Source name |
Unaffected biological mother of mentally retarded child 0621c (child).
|
| Organism |
Homo sapiens |
| Characteristics |
Tissue: whole blood
|
| Biomaterial provider |
Jan M Friedman
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated from whole blood using the Puregen Gentra® DNA Purification Kit following the manufacturer's protocol.
|
| Label |
Biotin with Streptavidin, R-phycoerythrin conjugate (SAPE).
|
| Label protocol |
Labeling was performed according to the Affymetrix GeneChip® Mapping 100K Assay Manual (www.affymetrix.com).
|
| |
|
| Hybridization protocol |
Hybridization, washes and staining were performed as described in the Affymetrix GeneChip® Mapping 100K Assay Manual (www.affymetrix.com). Samples were hybridized to GeneChip® Human Mapping 50K Xba240 or Hind240 arrays in an Affymetrix Hybridization Oven 640. Washes and staining of the arrays were performed with an Affymetrix Fluidics Station 450.
|
| Scan protocol |
Array images were obtained using an Affymetrix GeneChip® Scanner 3000, as described in the Affymetrix GeneChip® Mapping 100K Assay Manual (www.affymetrix.com).
|
| Description |
50K Hind Array data
|
| Data processing |
Mapping algorithm settings: Gtype(dm) heterozygote call threshold= 0.25, homozygote call threshold=0.25
|
| |
|
| Submission date |
Mar 08, 2007 |
| Last update date |
Mar 12, 2007 |
| Contact name |
BCGSC BC Cancer Agency |
| E-mail(s) |
[email protected]
|
| Organization name |
Canada's Michael Smith Genome Sciences Centre
|
| Department |
Gene Expression
|
| Lab |
Marco Marra
|
| Street address |
675 West 10th Avenue
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V5Z 1L3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL2004 |
| Series (1) |
| GSE7226 |
Oligonucleotide microarray analysis of genomic imbalance in children with mental retardation. |
|
