|
| Status |
Public on Mar 14, 2007 |
| Title |
GM10860_technical_rep_2 |
| Sample type |
RNA |
| |
|
| Source name |
B lymphoblastoid cell line
|
| Organism |
Homo sapiens |
| Characteristics |
CEPH
|
| Biomaterial provider |
Coriell Cell Repositories http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM10860
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Following fragmentation, 10 microg of cRNA was hybridized for 16 hr at 45C on GeneChip Human Focus Array.
|
| |
|
| Hybridization protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol at the UW Center for Expression Arrays.
|
| Scan protocol |
Arrays were scanned using the GeneChip Scanner 3000.
|
| Description |
Gene expression data from B lymphoblastoid cell lines grown according to Coriell instructions.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Mar 09, 2007 |
| Last update date |
Aug 28, 2007 |
| Contact name |
Joshua M Akey |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Washington
|
| Department |
Genome Sciences
|
| Lab |
Akey
|
| Street address |
1705 NE Pacific St
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195 |
| Country |
USA |
| |
|
| Platform ID |
GPL201 |
| Series (1) |
| GSE7236 |
Natural gene expression variation in the HapMap CEU and YRI samples |
|
