|
Status |
Public on Feb 20, 2008 |
Title |
T9 BAL Urethane Treated |
Sample type |
RNA |
|
|
Source name |
A/J mouse (Jackson Labs) bronchoalveolar lavage cells
|
Organism |
Mus musculus |
Characteristics |
Age matched Control, no treatment
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 to 5 microg total RNA
|
|
|
Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip MOE430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
Description |
Gene expression data from normal mouse lung tissue
|
Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method arbitrarily set to 500
|
|
|
Submission date |
Mar 13, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Robert S Stearman |
E-mail(s) |
[email protected]
|
Phone |
303-315-2317
|
Fax |
303-315-5632
|
Organization name |
UCHSC
|
Department |
Medicine/Pulmonary
|
Lab |
Stearman
|
Street address |
4200 E Ninth Ave
|
City |
Denver |
State/province |
CO |
ZIP/Postal code |
80262 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (2) |
GSE7258 |
Expression data of bronchoalveolar lavage cells from control or urethane treated AJ mice |
GSE7269 |
AJ mouse lung carcinogenesis study |
|
Relations |
Reanalyzed by |
GSE119085 |