|
| Status |
Public on Mar 28, 2007 |
| Title |
Terminal duct lobular units (S04-21945-TDLU) |
| Sample type |
RNA |
| |
|
| Source name |
non-cancerous adult female human breast
|
| Organism |
Homo sapiens |
| Characteristics |
Formalin-fixed paraffin-embedded tissue (FFPET) biopsies containing paired normal TDLUs and well-developed HELUs from non-cancerous adult female human breasts obtained within the past 3 years
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using the Optimum Kit, which is based on proteinase K digestion followed by phase separation in a proprietary micro-filter cartridge and incubation with DNase to remove contaminating genomic DNA.
|
| Label |
biotin
|
| Label protocol |
The cDNA was in vitro transcribed to biotin-labeled cRNA using IVT Labeling Kit.
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 mg cRNA were hybridized to the microarrays for 16 hours at 45C.
|
| Scan protocol |
The chips were washed in a fluidic station and the phycoerythrin-stained array was scanned as a digital image file as described in the Affymetrix GeneChip protocol
|
| Description |
Gene expression data from benign hyperplastic breast epithelial cells
|
| Data processing |
Data was processed and analyzed with GCOS 1.2 using affymetrix default settings.
|
| |
|
| Submission date |
Mar 27, 2007 |
| Last update date |
Mar 27, 2007 |
| Contact name |
Sangjun Lee |
| E-mail(s) |
[email protected]
|
| Phone |
314-454-5119
|
| Fax |
314-454-5505
|
| Organization name |
Washington University at St. Louis
|
| Department |
Pathology
|
| Lab |
Dr. Allred
|
| Street address |
4940 Parkview Place
|
| City |
St Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL1352 |
| Series (1) |
| GSE7377 |
Expression data from premalignant breast cancers |
|
