|
| Status |
Public on Jun 11, 2007 |
| Title |
VDXRHU_329 |
| Sample type |
RNA |
| |
|
| Source name |
VDXRHU_329
|
| Organism |
Homo sapiens |
| Characteristics |
samplename: VDXRHU_329
id: 329
filename: 159-329_RHA.CEL.gz
hospital: RH
age: 60
size: 3
Surgery_type: 1
Histtype: 4
Angioinv: 0
Lymp_infil: NA
node: 0
grade: 2
er: 1
t.rfs: 6281
e.rfs: 0
t.os: 6281
e.os: 0
t.dmfs: 6281
e.dmfs: 0
t.tdm: 6281
e.tdm: 0
risksg: 2
NPI: 3.6
risknpi: 2
AOL_os_10y: 73.4
risk_AOL: 1
veridex_risk: Poor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Isolation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc)olation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc).
|
| Label |
biotin
|
| Label protocol |
Approximately 1 microgram of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures.
|
| Scan protocol |
standard Affymetrix procedures.
|
| Description |
n/a
|
| Data processing |
MAS5 Bioconductor (target value of 600).
|
| |
|
| Submission date |
Mar 28, 2007 |
| Last update date |
Jun 11, 2007 |
| Contact name |
Benjamin Haibe-Kains |
| E-mail(s) |
[email protected]
|
| Phone |
+14165818626
|
| Organization name |
Princess Margaret Cancer Centre
|
| Department |
Princess Margaret Research
|
| Lab |
Bioinformatics and Computational Genomics
|
| Street address |
610 University Avenue
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 2M9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE7390 |
Strong Time Dependence of the 76-Gene Prognostic Signature |
|
