|
| Status |
Public on Mar 01, 2008 |
| Title |
U0126_PDGF_half_hour, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
SH-SY5Y cells treated with 50 mM U0126 for 1h, then with 50ng/ml PDGF for 0.5h.
|
| Organism |
Homo sapiens |
| Characteristics |
SH-SY5Y neuroblastoma cells
SH-SY5Y cells treated with 50 mM U0126 for 1h, then with 50ng/ml PDGF for 0.5h.
|
| Treatment protocol |
SH-SY5Y cells treated with 50 mM U0126 for 1h, then with 50ng/ml PDGF for 0.5h.
|
| Growth protocol |
SH-SY5Y cells were grown to confluence and starved overnight in serum-free medium in order to silence any sustained effects from growth factor signaling.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated 0.5 hours after PDGF addition. Experiments were performed in duplicate. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from SH-SY5Y cells treated with 50 mM U0126 for 1h, then with 50ng/ml PDGF for 0.5h.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
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| |
|
| Submission date |
Mar 29, 2007 |
| Last update date |
Feb 27, 2008 |
| Contact name |
Alena A Antipova |
| Organization name |
Broad Institute of Massachusetts Institute of Technology and Harvard University
|
| Street address |
7 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE7403 |
Expression data from PDGF-treated SH-SY5Y cells |
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