|
| Status |
Public on Apr 05, 2007 |
| Title |
U-2OS (decitabine treated) expression profile (AffyChip U133 Plus 2.0 platform) |
| Sample type |
RNA |
| |
|
| Source name |
U-2OS cell line from ATCC
|
| Organism |
Homo sapiens |
| Characteristics |
human bone tumor cell line treated with 1 uM decitabine for 3 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAeasy kit from Qiagen
|
| Label |
Biotin
|
| Label protocol |
Total RNA was extracted using the RNeasy kit (Qiagen, Germany) from U-2OS cells at Day3 after treatment with 1 µM decitabine or medium alone (control). In each experiment RNA yields were pooled from two independent cultures per treatment arm to minimize experimental noise. For each case, 10 µg of RNA was labeled using the two cycle target labeling assays as described by Affymetrix manual.
|
| |
|
| Hybridization protocol |
For each case, 10 µg of RNA was labeled and hybridized to the Affymetrix HG-U133A GeneChips using the manufacturer’s protocol (Affymetrix, Santa Clara, CA) by the Center of Applied Genomics at the Hospital for Sick Children (Toronto, Canada).
|
| Scan protocol |
Data were extracted using the Microarray Suite (MAS) version 5.0 (Affymetrix) and linearly scaled to achieve an average intensity of 150 across each chip.
|
| Description |
non
|
| Data processing |
Data were extracted using the Microarray Suite (MAS) version 5.0 (Affymetrix) and linearly scaled to achieve an average intensity of 150 across each chip.
|
| |
|
| Submission date |
Apr 04, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Khaldoun Al-Romaih |
| E-mail(s) |
[email protected]
|
| Phone |
416 946 4509
|
| Organization name |
Ontario Cancer Institute
|
| Department |
AMO
|
| Lab |
Dr Squire Lab
|
| Street address |
610 University Ave
|
| City |
Toronot |
| ZIP/Postal code |
M5G 2M9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE7454 |
Modulation of gene expression by decitabine in U-2OS cells in vitro and in vivo |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
