|
| Status |
Public on May 31, 2007 |
| Title |
Adenoma patient 125 |
| Sample type |
RNA |
| |
|
| Source name |
Adenoma patient 125
|
| Organism |
Homo sapiens |
| Characteristics |
benign
Histology:Serous cystadenoma
Stage:na
Age at surgery: 61
|
| Treatment protocol |
Tissue was collected at the time of surgery and preserved in RNAlater (Ambion, Austin, TX) within one minute of collection. Linear polyacrylamide (5 ul) was added to ~50 mg of tumor sample and homogenization was carried out on ice in 1.5 ml Trizol (Invitrogen, Carlsbad, CA) with a polytron homogenizer for 30 seconds.
|
| Growth protocol |
A set of 43 ovarian tumors was obtained from the Ovarian Cancer Institute (Atlanta). Tissue was collected at the time of surgery and preserved in RNAlater (Ambion, Austin, TX) within one minute of collection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from crude homogenate according to the manufacturer’s protocols (Trizol) and further enriched using an RNEasy column (Qiagen, Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using GeneArray Scanner Model G2500
|
| Description |
Gene expression data from ovarian adenoma.
|
| Data processing |
The data were analyzed using GCRMA normalization with GeneTraffic Software (Iobion, La Jolla, CA).
|
| |
|
| Submission date |
Apr 05, 2007 |
| Last update date |
Mar 16, 2009 |
| Contact name |
Nathan J. Bowen |
| E-mail(s) |
[email protected]
|
| Phone |
4048808158
|
| Organization name |
Clark Atlanta University
|
| Department |
Center for Cancer Research and Therapeutic Development
|
| Lab |
Cancer Development and Evolution
|
| Street address |
223 James P Brawley Drive
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30314 |
| Country |
USA |
| |
|
| Platform ID |
GPL8300 |
| Series (1) |
| GSE7463 |
Expression data from 43 Ovarian tumors |
|
