|
| Status |
Public on Nov 03, 2015 |
| Title |
HDM sensitized atopic subject # AA09 medium control |
| Sample type |
RNA |
| |
|
| Source name |
CD4 T cells isolated from PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
cell population: CD4 T cell
igg: IgE ratio: 13.837984
|
| Treatment protocol |
PBMC were cultured in medium alone or in the presence of HDM extract (10 ug/ml).
At the termination of the cultures, CD4 T cells were isolated using immunomagnetic separation.
|
| Growth protocol |
PBMC were cultured in AIM-V medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted employing TRIzol followed by RNeasy.
|
| Label |
Biotin
|
| Label protocol |
The samples were labelled using standardized kits from Affymetrix at the Ramaciotti Centre for Genomics, The University of New South Wales.
|
| |
|
| Hybridization protocol |
The samples were hybridized to Human Gene ST1.0 microarrays according to standard Affymetrix protocols. This was performed by at the Ramaciotti Centre for Genomics, The University of New South Wales.
|
| Scan protocol |
The samples were scanned on an Affymetrix scanner according to standard Affymetrix protocols at the Ramaciotti Centre for Genomics, The University of New South Wales.
|
| Data processing |
The microarray data was preprocessed in R employing the RMA algorithm and a custom chip description file (hugene10st_Hs_ENTREZG, v19). ComBat was used to adjust the data for batch effects.
|
| |
|
| Submission date |
Jul 10, 2015 |
| Last update date |
Nov 03, 2015 |
| Contact name |
Anthony Bosco |
| E-mail(s) |
[email protected]
|
| Organization name |
Telethon Kids Institute
|
| Street address |
100 Roberts Road
|
| City |
Subiaco |
| State/province |
WA |
| ZIP/Postal code |
6008 |
| Country |
Australia |
| |
|
| Platform ID |
GPL20171 |
| Series (1) |
| GSE70760 |
Gene expression patterns in house dust mite stimulated CD4 T cells and IgG:IgE ratios |
|
