|
| Status |
Public on Apr 13, 2007 |
| Title |
RCG-12 cells cultured at permissive temperature for 3 days-1 |
| Sample type |
RNA |
| |
|
| Source name |
Rat astrocyte cell line RCG-12
|
| Organism |
Rattus norvegicus |
| Characteristics |
Rat astrocyte cell line RCG-12
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the cells using an RNeasy Total RNA Extraction Kit (Qiagen K.K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
|
| Label |
phycoerythrin
|
| Label protocol |
5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
|
| |
|
| Hybridization protocol |
After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
|
| Scan protocol |
The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
|
| Description |
A rat conditionally immortalized astrocyte cell line, RCG-12, derived from primary cultured rat cortical glia cells infecting with a temperature-sensitive simian virus 40 large T-antigen was used. RCG-12 cells were cultured at permissive temperature (33°C) for 3 days.
|
| Data processing |
GeneChip Analysis Suite
|
| |
|
| Submission date |
Apr 12, 2007 |
| Last update date |
Apr 12, 2007 |
| Contact name |
Yoshiaki Tabuchi |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Toyama
|
| Department |
Life Science Research Center
|
| Lab |
Division of Molecular Genetics Research
|
| Street address |
2630 Sugitani
|
| City |
Toyama |
| State/province |
Toyama |
| ZIP/Postal code |
930-0194 |
| Country |
Japan |
| |
|
| Platform ID |
GPL341 |
| Series (1) |
| GSE7501 |
Genes in nonpermissive temperature-induced cell growth arrest and differentiation of astrocyte RCG-12 cells |
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