|
| Status |
Public on Jul 21, 2015 |
| Title |
H2R2 72679_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
H2R2 72679
|
| Organism |
Gallus gallus |
| Characteristics |
tissue: Small yellow follicles
strain: B strain Taiwan country chicken
age: 30 wk
stress: acute heat stress (38℃) for 2 h
time (recovery): 2 hr
|
| Treatment protocol |
Hens were treated to acute heat stress (38℃) for 2 h, and recovered at 25℃ for 0, 2, and 6 h. Control goup was non-heat stressed chicken which stayed at 25℃.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
0.2 μg of total RNA was amplified by a Low Input Quick-Amp Labeling kit (Agilent Technologies, USA) and labeled with Cy3 (CyDye, Agilent Technologies, USA) during the in vitro transcription process.
|
| Label |
Cy3
|
| Label protocol |
1.65 μg of Cy3-labled cRNA was fragmented to an average size of about 50-100 nucleotides by incubation with fragmentation buffer at 60°C for 30 minutes.
|
| |
|
| Hybridization protocol |
Correspondingly fragmented labeled cRNA is then pooled and hybridized to Agilent chicken V2 Oligo 4×44K Microarray (Agilent Technologies, USA) at 65°C for 17 h.
|
| Scan protocol |
After washing and drying by nitrogen gun blowing, microarrays are scanned with an Agilent microarray scanner (Agilent Technologies, USA) at 535 nm for Cy3.
|
| Description |
Gene expression at 2 h of recovery after acute heat stress
17598
|
| Data processing |
Scanned images are analyzed by Feature extraction10.5.1.1 software (Agilent Technologies, USA), an image analysis and normalization software is used to quantify signal and background intensity for each feature.
|
| |
|
| Submission date |
Jul 20, 2015 |
| Last update date |
Jul 21, 2015 |
| Contact name |
San Yuan Huang |
| E-mail(s) |
[email protected]
|
| Phone |
886-4-22870613-245
|
| Organization name |
National Chung Hsing University
|
| Department |
Animal science
|
| Lab |
Animal proteomics
|
| Street address |
Kuo-Kuang Rd.
|
| City |
Taichung |
| ZIP/Postal code |
402 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL15357 |
| Series (1) |
| GSE71091 |
Acute heat stress induces differential gene expressions in the small yellow follicles of a broiler-type strain of Taiwan country chickens |
|
