|
| Status |
Public on Jul 21, 2015 |
| Title |
BMP7_2 |
| Sample type |
RNA |
| |
|
| Source name |
C3H10T1/2 BMP7 treatment
|
| Organism |
Mus musculus |
| Characteristics |
cell line: C3H10T1/2
|
| Treatment protocol |
C3H10T1/2 cells were treated with 3.3 BMP7 or vehicle for 3 days
|
| Growth protocol |
C3H10T1/2 cells on culture dishes were grown to 100% confluency
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cells using ULTRASPEC RNA isolation system (Bioteecx Laboratories) and followed by Rneasy Mini columns (Qiagen) purification.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA following the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
15 ug of adjusted cRNA were hybridized tp Affymetric GeneChip Mouse 430A2.0 Array. GeneChips were washed and stained using the protocols provided by the manuscripture.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
Gene expression data from C3H10T1/2 cells treated with BMP7 vs vehicle
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. All chips were subjected to global scaling to a target intensity of 1500 to take into account the inherent differences between the chips and their hybridization efficiencies.
|
| |
|
| Submission date |
Jul 20, 2015 |
| Last update date |
Jul 21, 2015 |
| Contact name |
Yu-Hua Tseng |
| E-mail(s) |
[email protected]
|
| Organization name |
Joslin Diabetes Center Harvard Medical School
|
| Street address |
1 Joslin Place
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE71101 |
Expression data from C3H10T1/2 cells treated with 3.3 nM BMP7 vs vehicle |
|
