|
| Status |
Public on May 23, 2016 |
| Title |
Anterior-3 |
| Sample type |
RNA |
| |
|
| Source name |
Wing discs, anterior compartment cells
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain/background: w1118
developmental stage: 8-day-old larvae
tissue: wing imaginal discs
cell population: RFP-negative, MMP1-GFP-negative
|
| Treatment protocol |
Drosophila wing imaginal discs were dissected, dissociated with trypsin and cells were sorted by FACS.
|
| Growth protocol |
Drosophila larvae expressing p35, a dsRNA form of asp and RFP under the control of the en-gal4 driver and carrying an MMP1-GFP reporter construct were grown until ~8 days old.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction and cDNA amplification were performed as described in Gonzalez-Roca E et al. (Plos One, 2010). cDNA library was amplified using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2).
|
| Label |
biotin
|
| Label protocol |
Amplified cDNA was labeled using GeneChip Mapping 250K Nsp Assay Kit (Affymetrix; catalog # 900766), according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Affymetrix Drosophila Genome 2.0 arrays were hybridized with 8 ug of labeled dsDNA, washed, stained and scanned according to the protocol described in 3’IVT express kit User Manual.
|
| Scan protocol |
Arrays scanning and CEL files generation were performed using an Affymetrix GeneChip Scanner 3000.
|
| Description |
Ant_3
Cells from the anterior compartment (RFP-negative, MMP1-GFP-negative).
|
| Data processing |
RMA normalization using the normqc function of Bioconductor's MDA package.
|
| |
|
| Submission date |
Jul 22, 2015 |
| Last update date |
May 23, 2016 |
| Contact name |
Camille Stephan Otto Attolini |
| Organization name |
IRB Barcelona
|
| Department |
Biostatistics and bioinformatics Unit
|
| Street address |
Baldiri Reixac 10
|
| City |
Barcelona |
| State/province |
Select a State or Province |
| ZIP/Postal code |
08028 |
| Country |
Spain |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE71242 |
Gene dosage imbalance contributes to chromosomal instability-induced tumorigenesis |
|
