|
| Status |
Public on Jul 31, 2007 |
| Title |
HeLa sample 1 |
| Sample type |
RNA |
| |
|
| Source name |
HeLa
|
| Organism |
Homo sapiens |
| Characteristics |
Cervical cancer cell line (HPV 18)
|
| Growth protocol |
cells were grown semiconfluent in RPMI 1640, supplemented with 10% FCS, streptomycin, and penicillin at 37ºC and 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix focus arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data
|
| Data processing |
The data files of all arrays were normalized using sequence information in Robust Multi_Chip Average (GCRMA) method.
|
| |
|
| Submission date |
Apr 19, 2007 |
| Last update date |
Jul 30, 2007 |
| Contact name |
Jan Oosting |
| Phone |
+31 71 5266619
|
| Organization name |
Leiden University Medical Center
|
| Department |
Pathology
|
| Street address |
Albinusdreef 2
|
| City |
Leiden |
| ZIP/Postal code |
2333ZA |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL201 |
| Series (1) |
| GSE8605 |
Combined arrayCGH and SNP-loss of heterozygosity analysis in cervical cancer |
|
