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Sample GSM1831900 Query DataSets for GSM1831900
Status Public on Oct 01, 2015
Title MCF7 Hotair +E2
Sample type RNA
 
Source name MCF7
Organism Homo sapiens
Characteristics cell line: MCF7
genotype/variation: HOTAIR
agent: 1 nM E2
Treatment protocol Lentiviral infected Ctrl or HOTAIR stable MCF7 cells were deprived for 3 days and then treated with ethanol or 1 nM E2 for 6h, cells were collected for RNA extraction.
Growth protocol Cells were maintained in DMEM supplemented with 10% FBS
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions. The quantity and quality of the isolated RNAs were determined using ND-1000 Spectrophotometer.
Label biotin
Label protocol The arrays were processed at University of Chicago Genomics Core (Illinois) according to manufacturer’s protocol.
 
Hybridization protocol The arrays were processed at University of Chicago Genomics Core (Illinois) according to manufacturer’s protocol.
Scan protocol The arrays were processed at University of Chicago Genomics Core (Illinois) according to manufacturer’s protocol.
Description SAMPLE 4
Data processing The data were processed and normalised using Illumina GenomeStudio
 
Submission date Jul 24, 2015
Last update date Oct 01, 2015
Contact name Jindan Yu
E-mail(s) [email protected]
Organization name Emory University
Department Urology
Lab Jindan Yu's lab
Street address E330, 1760 Haygood Dr NE
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
 
Platform ID GPL10558
Series (2)
GSE71297 LncRNA HOTAIR enhances ER signaling and confers tamoxifen resistance in breast cancer [array]
GSE71298 LncRNA HOTAIR enhances ER signaling and confers tamoxifen resistance in breast cancer

Data table header descriptions
ID_REF
VALUE background-subtracted, quantile-normalized expression value
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1774344 2.2 0.25714
ILMN_1712707 28.5 0
ILMN_1699750 -1 0.57403
ILMN_1739081 17.8 0
ILMN_2329958 108.8 0
ILMN_1775944 16.1 0
ILMN_1724497 128.9 0
ILMN_1720988 29.6 0
ILMN_1707274 -4.3 0.89351
ILMN_1755658 -1.8 0.68571
ILMN_1673639 0.4 0.42987
ILMN_1713732 179.4 0
ILMN_1708922 17.7 0
ILMN_2268241 21.7 0
ILMN_1741628 3 0.18961
ILMN_1657870 0.9 0.38182
ILMN_2240003 1.8 0.28571
ILMN_1765767 -0.3 0.51299
ILMN_1685703 89 0
ILMN_1743396 73.1 0

Total number of rows: 47317

Table truncated, full table size 1089 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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