|
Status |
Public on Oct 01, 2015 |
Title |
MCF7 Hotair +E2 |
Sample type |
RNA |
|
|
Source name |
MCF7
|
Organism |
Homo sapiens |
Characteristics |
cell line: MCF7 genotype/variation: HOTAIR agent: 1 nM E2
|
Treatment protocol |
Lentiviral infected Ctrl or HOTAIR stable MCF7 cells were deprived for 3 days and then treated with ethanol or 1 nM E2 for 6h, cells were collected for RNA extraction.
|
Growth protocol |
Cells were maintained in DMEM supplemented with 10% FBS
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions. The quantity and quality of the isolated RNAs were determined using ND-1000 Spectrophotometer.
|
Label |
biotin
|
Label protocol |
The arrays were processed at University of Chicago Genomics Core (Illinois) according to manufacturer’s protocol.
|
|
|
Hybridization protocol |
The arrays were processed at University of Chicago Genomics Core (Illinois) according to manufacturer’s protocol.
|
Scan protocol |
The arrays were processed at University of Chicago Genomics Core (Illinois) according to manufacturer’s protocol.
|
Description |
SAMPLE 4
|
Data processing |
The data were processed and normalised using Illumina GenomeStudio
|
|
|
Submission date |
Jul 24, 2015 |
Last update date |
Oct 01, 2015 |
Contact name |
Jindan Yu |
E-mail(s) |
[email protected]
|
Organization name |
Emory University
|
Department |
Urology
|
Lab |
Jindan Yu's lab
|
Street address |
E330, 1760 Haygood Dr NE
|
City |
Atlanta |
State/province |
GA |
ZIP/Postal code |
30322 |
Country |
USA |
|
|
Platform ID |
GPL10558 |
Series (2) |
GSE71297 |
LncRNA HOTAIR enhances ER signaling and confers tamoxifen resistance in breast cancer [array] |
GSE71298 |
LncRNA HOTAIR enhances ER signaling and confers tamoxifen resistance in breast cancer |
|