|
| Status |
Public on May 28, 2008 |
| Title |
Group 3, normal human epidermal melanocytes, NHEM |
| Sample type |
RNA |
| |
|
| Source name |
cultured normal human epidermal melanocytes purchased from Cambrex BioScience
|
| Organism |
Homo sapiens |
| Characteristics |
Group 3
normal human epidermal melanocytes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA was hybridized for 16 hr at 45C on GeneChip 133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCS3000 Scanner.
|
| Description |
Gene expression data from normal Human melanocytes cultured in vitro
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Apr 19, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Sean Yoder |
| E-mail(s) |
[email protected]
|
| Phone |
813-745-1917
|
| Organization name |
Moffitt Cancer Center & Research Institute
|
| Department |
Molecular Genomics Core Facility
|
| Lab |
SRB-2
|
| Street address |
12902 Magnolia Dr.
|
| City |
Tampa |
| State/province |
FL |
| ZIP/Postal code |
33612 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE7553 |
Gene Expression Patterns Involved in the Malignant Transformation and Progression of Metastatic Melanoma |
|
| Relations |
| Reanalyzed by |
GSE119087 |
