|
| Status |
Public on Jan 09, 2008 |
| Title |
White adipose tissue, fed #4 |
| Sample type |
RNA |
| |
|
| Source name |
white adipose tissue
|
| Organism |
Rattus norvegicus |
| Characteristics |
Strain: Wistar
Gender: male
Age: 8 weeks
Tissue: perinephrial white adipose tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolatedusing ISOGEN (Nippon Gene Co., Ltd., Toyama, Japan), then purified with an RNeasy mini kit (Qiagen K.K., Tokyo, Japan).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNAs were prepared according to the standard Affymetrix protocol from 2 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 µg cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
|
| Description |
No additional information
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Apr 25, 2007 |
| Last update date |
Jan 09, 2008 |
| Contact name |
Yuji Nakai |
| Organization name |
Hirosaki University
|
| Department |
Institute of Regional Innovation
|
| Lab |
Section of Food Sciences
|
| Street address |
2-1-1, Yanagawa
|
| City |
Aomori |
| State/province |
Aomori |
| ZIP/Postal code |
038-0012 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE7623 |
24 h-fasting effects on the brown and white adipose tissue and liver |
|
