|
| Status |
Public on Jul 15, 2008 |
| Title |
Sedentary rat 3 |
| Sample type |
RNA |
| |
|
| Source name |
Left ventricle of sedentary rat
|
| Organism |
Rattus norvegicus |
| Characteristics |
Sprague-Dawley-CD rat (8 weeks old, 360±15 g body weight at the beginning of the study)
|
| Growth protocol |
We used 20 male Sprague–Dawley-CD rats (8 weeks old, 360±15 g body weight at the beginning of the study), randomly divided into two groups: sedentary- controls and exercise-trained animals. During the experimental period, all animals had free access to water and conventional laboratory diet (Standard diet n.48, Laboratorio Dottori Piccioni,Gessate, Milan, Italy) until sacrifice. Room temperature was kept at 21 ± 2°C and 12 h of light were automatically alternated to 12 h of dark.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug of total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
Gene expression data from left ventricle of sedentary rat
|
| Data processing |
The data were analyzed with Bioconductor Packages.
|
| |
|
| Submission date |
Apr 26, 2007 |
| Last update date |
May 16, 2008 |
| Contact name |
Betti Giusti |
| Organization name |
University of Florence
|
| Department |
Dpt. of Medical and Surgical Critical Care
|
| Street address |
viale Morgagni, 85
|
| City |
Florence |
| ZIP/Postal code |
50134 |
| Country |
Italy |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE7640 |
Gene expression profile induced by moderate physical exercise in heart left ventricles in rats |
|
