|
| Status |
Public on Aug 30, 2007 |
| Title |
CL1-5-F4: Adenocarcinoma |
| Sample type |
RNA |
| |
|
| Source name |
CL1-5-F4
|
| Organism |
Homo sapiens |
| Characteristics |
Adenocarcinoma
sex: male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA preparation and analysis were performed according to the Affymetrix's instructions. The quality of the total RNA for microarray analysis was determined using Spectra Max Plus (Molecular Devices) and had an OD260/OD280 ratio ranging from 1.9 to 2.1.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Protocols and reagents for hybridization, washing and staining followed the Affymetrix’s instructions (http://www.affymetrix.com/support/technical/manuals.affx).
|
| Scan protocol |
GeneChips were scanned using the Affemetrix Genechip scanner 6G.
|
| Description |
Gene expression data from cell line.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Apr 30, 2007 |
| Last update date |
Aug 30, 2007 |
| Contact name |
Li-Jen Su |
| E-mail(s) |
[email protected]
|
| Organization name |
Taipei Veterans General Hospital
|
| Department |
Department of Surgery
|
| Street address |
No. 201, Sec. 2, Shih-Pai Road
|
| City |
Taipei |
| ZIP/Postal code |
112 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE7670 |
Expression data from Lung cancer |
|
