|
| Status |
Public on May 23, 2007 |
| Title |
T8 mglA A |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Amplified RNA from bone marrow derived macrophages from female mice 2 months old C57/bl6
|
| Organism |
Mus musculus |
| Characteristics |
Amplified RNA from bone marrow derived macrophages
|
| Extracted molecule |
total RNA |
| Extraction protocol |
1000000 macrophages were lysed in 1mL Trizol and flashed frozen in liquid nitrogen. Total RNA was extracted with chloroform, followed by Ethanol precipitation and purification using RNeasy kit (Qiagen USA, Valencia, CA)"
|
| Label |
Cy5
|
| Label protocol |
RNA extracted from the samples was amplified using Amino Allyl MessageAmp II aRNA kit from Ambion. The amplified material was then labeled with Cy5 using cyTM dye Post-labelling Reactive Dye pack (Amersham)."
|
| |
|
| Channel 2 |
| Source name |
mouse universal reference RNA (Stratagene)
|
| Organism |
Mus musculus |
| Characteristics |
Universal RNA reference pool
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from the Mouse Universal reference (Stratagene)
|
| Label |
Cy3
|
| Label protocol |
RNA from the Mouse Universal reference (Stratagene) was amplified using Amino Allyl MessageAmp II aRNA kit from Ambion. The amplified material was then labeled with Cy3 using cyTM dye Post-labelling Reactive Dye pack (Amersham)?
|
| |
|
| |
| Hybridization protocol |
Cy5 labeled sample was hybridized to the MEEBO array in 3 SSC at 63C 36 hours."
|
| Scan protocol |
MEEBO arrays were scanned with an Axon 4000B scanner (Axon Instruments, Union City, CA, USA) and gridded using the bundled GenePix 3.0 software."
|
| Description |
T8 mglA A
|
| Data processing |
Spot analysis was performed with GenePix v3.0 and stored in a relational database (NOMAD).
|
| |
|
| Submission date |
May 01, 2007 |
| Last update date |
May 22, 2007 |
| Contact name |
Sajeev Batra |
| Organization name |
UCSF
|
| Street address |
Box 2240, 600 16th Street S474
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platform ID |
GPL5137 |
| Series (1) |
| GSE7684 |
Type I interferon signaling is required for activation of the inflammasome during Francisella infection |
|
