|
| Status |
Public on Sep 02, 2015 |
| Title |
Macrophages treated with ManLAM (replicate) vs. untreated 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Macrophages treated with ManLAM
|
| Organism |
Mus musculus |
| Characteristics |
cell line: J774.1
treatment: ManLAM
|
| Treatment protocol |
Cells were treated with ManLAM (5 ug/ml) either in the absence or in the presence of GW9662 for 6 h, washed and RNA was isolated.
|
| Growth protocol |
J774A.1 cells were grown in DMEM supplemented with 10% FBS at 37 degrees C in 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the Ambion miRVana RNA isolation kit.
|
| Label |
Cy5
|
| Label protocol |
T7 RNA polymerase amplification to generate labeled complementary RNA. Treated: Cy5; Untreated: Cy3
|
| |
|
| Channel 2 |
| Source name |
Untreated J774A.1 cells
|
| Organism |
Mus musculus |
| Characteristics |
cell line: J774A.1
treatment: none
|
| Treatment protocol |
Cells were treated with ManLAM (5 ug/ml) either in the absence or in the presence of GW9662 for 6 h, washed and RNA was isolated.
|
| Growth protocol |
J774A.1 cells were grown in DMEM supplemented with 10% FBS at 37 degrees C in 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the Ambion miRVana RNA isolation kit.
|
| Label |
Cy3
|
| Label protocol |
T7 RNA polymerase amplification to generate labeled complementary RNA. Treated: Cy5; Untreated: Cy3
|
| |
|
| |
| Hybridization protocol |
Agilent's In situ Microarray Hybridization Chamber Kit (G2534A)
|
| Scan protocol |
Microarray slides were scanned using an Agilent microarray scanner at 3 micron resolution.
|
| Description |
J774.1 cell treated with ManLAM
|
| Data processing |
Microarray data was normalized and analysed using using GeneSpring GX version 11.5 and Microsoft Excel.
|
| |
|
| Submission date |
Sep 01, 2015 |
| Last update date |
Sep 02, 2015 |
| Contact name |
Joyoti Basu |
| Organization name |
Bose Institute
|
| Department |
Chemistry
|
| Street address |
A.P.C. Road
|
| City |
Kolkata |
| ZIP/Postal code |
700009 |
| Country |
India |
| |
|
| Platform ID |
GPL13381 |
| Series (1) |
| GSE72591 |
Time-dependent global gene expression profile of J774A.1 cells treated with ManLAM in the absence or presence of GW9662 |
|
