|
| Status |
Public on Sep 02, 2016 |
| Title |
group 1, no IPC and 90 minutes warm ischemia 2 |
| Sample type |
RNA |
| |
|
| Source name |
kidney_72 hr_without IPC
|
| Organism |
Sus scrofa |
| Characteristics |
breed: Yorkshire
gender: female
sample group: no IPC and 90 min warm ischemia
tissue: kidney
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from tissues using TRIzol (Invitrogen, Carlsbad, CA), in accordance with the manufacturer's protocol, and purified by RNeasy Mini Kit (Qiagen, Valencia, CA)
|
| Label |
Cy3
|
| Label protocol |
labeled cRNA were prepared from 1~5 ug total RNA using the Agilent’s Quick Amp Labeling Kit
|
| |
|
| Hybridization protocol |
Following fragmentation, 1.65 ug of cRNA were hybridized to the Agilent expression microarray according to the protocols provided by the manufacturer
|
| Scan protocol |
Arrays were scanned using the Agilent Technologies G2600D SG12494263
|
| Description |
SAMPLE 2
|
| Data processing |
Array data export processing and analysis was performed using Agilent Feature Extraction v11.0.1.1
|
| |
|
| Submission date |
Sep 02, 2015 |
| Last update date |
Sep 02, 2016 |
| Contact name |
Young Eun Yoon |
| Organization name |
Yonsei University
|
| Department |
Urology
|
| Street address |
yonsei-ro 50-1
|
| City |
Seoul |
| ZIP/Postal code |
120-752 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL16571 |
| Series (1) |
| GSE72646 |
The analysis of differential gene expression after remote ischemic preconditioning of renal ischemia reperfusion injury |
|
