|
| Status |
Public on Aug 15, 2007 |
| Title |
CF Sputum pool isolate minimal media citrate replicate1 |
| Sample type |
RNA |
| |
|
| Source name |
CF Sputum pool isolate minimal media citrate replicate1
|
| Organism |
Pseudomonas aeruginosa |
| Characteristics |
A pool of Pseudomonas aeruignosa was purified from CF sputum patient 42 and grown in minimal media with 20 mM citrate. This is our in vitro grown sample.
|
| Biomaterial provider |
Dr. Wallace J. Matthews
|
| Treatment protocol |
Sputum samples were maintained on ice at all times. Sputum was treated with Sputolysin, proteinase K, DNase I, and pelleted. Cell pellet was washed twice with COLD double distilled water and a 100 ul aliquot plated on PIA media for generation of a pool to be stored for in vitro studies. Colonies were pooled and grown for 1 hr in PIA and then frozen away at -80 in small 250 ul aliquots. One aliquot was used for each GeneChip study.
|
| Growth protocol |
1x M9 minimal media supplemented with 20 mM citrate
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Pseudomonas aeruginosa total RNA isolation protocol developed by S. Lory, and cleanded up with QIAgen RNeasy with extra oncolumn DNase I treatment
|
| Label |
Biotin
|
| Label protocol |
As per Affymetrix protocol for terminal labeling (used EnzoArray)
|
| |
|
| Hybridization protocol |
As per Affymetrix protocol
|
| Scan protocol |
As per Affymetrix protocol
|
| Description |
Samples processed at Greenwood Biotechnology Facility (Honolulu, HI)
|
| Data processing |
Signal Values determined using MAS5
|
| |
|
| Submission date |
May 03, 2007 |
| Last update date |
Jun 21, 2007 |
| Contact name |
Tung Hoang |
| E-mail(s) |
[email protected]
|
| Phone |
808-956-5035
|
| Organization name |
University of Hawaii at Manoa
|
| Department |
Microbiology
|
| Lab |
LSB 318
|
| Street address |
1800 East-West Road
|
| City |
Honolulu |
| State/province |
HI |
| ZIP/Postal code |
96822 |
| Country |
USA |
| |
|
| Platform ID |
GPL84 |
| Series (1) |
| GSE7704 |
In vivo evidence of Pseudomonas aeruignosa nutrient acquisition and pathogenesis in the cystic fibrosis lung |
|
