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Status |
Public on Sep 15, 2016 |
Title |
FKB_2 |
Sample type |
RNA |
|
|
Source name |
HeLa Cells, 48h FKB-Treated, replicate 2
|
Organism |
Homo sapiens |
Characteristics |
cell line: HeLa treatment: 48 hours of treatment with FKB: Flavokawain B
|
Treatment protocol |
FKB-treated cells were treated with 7µg/mL of FKB for 48 hours, FIH-treated cells were treated with 15 µg/mL of FIH for 48 hours, the untreated cells were treated with the vehicle.
|
Growth protocol |
HeLa cells were grown in RPMI-1640 supplemented with 10%FBS and 1% pennicillin-streptomycin and kept in a humidified 37 C chamber equipped with 5%CO2
|
Extracted molecule |
total RNA |
Extraction protocol |
Cells were extracted using the QIAGEN Rneasy Mini Kit as per manufacturer's protocol
|
Label |
Cy3
|
Label protocol |
Cy3labeled cRNA was prepared from 25 ng of total RNA using the Low Input Quick Amp Labelinh One-Color Microarray-Based Gene Expression Analysis
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|
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Hybridization protocol |
600 ng of Cy3-labeled cRNA (specific activity >6 pmol Cy3/µg cRNA) were fragemnted at 60C for 30 minutes in reaction volume of 25 µl with 1x Agilent Fragmentation Buffer and 2x Agilent blocking agent per manufacturer's protocol
|
Scan protocol |
slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (SureScan Microarray Scanner with the product number: G4900DA) using one color scan setting for 8X60k with scan area 61x21.6 mm and 3µm resolution. We used one color channel for Cy3
|
Description |
Gene expression in HeLa cells after 48 hours of treatment with FKB
|
Data processing |
The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) using default parameters (protocol GE1-v1_91 and Grid: 012391_D_20060331) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
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Submission date |
Sep 13, 2015 |
Last update date |
Apr 23, 2018 |
Contact name |
Nadiah Abu |
E-mail(s) |
[email protected]
|
Phone |
+60135103138
|
Organization name |
Universiti Putra Malaysia
|
Department |
Faculty of Biotechnology
|
Street address |
Animal Tissue Culture Lab
|
City |
Serdang |
State/province |
Selangor |
ZIP/Postal code |
43400 |
Country |
Malaysia |
|
|
Platform ID |
GPL17077 |
Series (1) |
GSE72974 |
Gene expression analysis reveals the concurrent activation of pro-apoptotic and antioxidant-defensive mechanisms in Flavokawain B and FIH-treated cervical cancer HeLa cells |
|
Relations |
Reanalyzed by |
GSE113533 |