|
| Status |
Public on May 15, 2007 |
| Title |
Murine_NK_IL-15_24h_1 |
| Sample type |
RNA |
| |
|
| Source name |
Splenic NK cells, IL-15 activated for 24 hours
|
| Organism |
Mus musculus |
| Characteristics |
C57Bl/6
|
| Biomaterial provider |
Jackson Labs
|
| Treatment protocol |
Splenic NK cells were flow sorted to >99% NK1.1+CD3- purity, stimulated with 100 ng/mL of IL-15 and then lysed in Trizol
|
| Growth protocol |
NK cells were stimulated with 100 ng/mL of rmIL-15
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol, per manufacturers protocol
|
| Label |
Biotinylated aRNA
|
| Label protocol |
Two cycle amplification by standard Affymetrix procedure
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
Murine NK cell were purified from wild type C57Bl/6 mice (>99% NK1.1+CD3-), stimulated with rmIL-15 for 24 hours, and then lysed in Trizol and assessed for gene expression on Affy MOE430_2 microarray
|
| Data processing |
Transformation procedure used:GCOS 1.4
|
| |
|
| Submission date |
May 08, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Rekha Meyer |
| E-mail(s) |
[email protected]
|
| Phone |
314 362 8853
|
| Organization name |
Washington University School of Medicine
|
| Street address |
4320 Forest Park Av
|
| City |
St Louis |
| State/province |
MO |
| ZIP/Postal code |
63128 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE7764 |
mRNA expression profiles of resting and IL-15 activated murine NK cells |
|
| Relations |
| Reanalyzed by |
GSE55732 |
| Reanalyzed by |
GSE56952 |
| Reanalyzed by |
GSE119085 |
