|
| Status |
Public on Sep 23, 2015 |
| Title |
cell line R7 at P2 rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila cell line R7 at Passage 2
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell line: R7
passage: 2
|
| Treatment protocol |
samples were collected from cells cultured in 25 cm2 T flasks
|
| Growth protocol |
cells cultured in Schneider's media supplemented with 10% FBS (fetal bovine serum) and antibiotics.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolation using All Prep RNA Qiagen.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (GeneChip 3' IVT Express Kit, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized to the Affymetrix Drosophila Genome 2.0 Array according to the protocols provided by the manufacturer.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from cell line R7 at Passage 2
|
| Data processing |
The data were analyzed using dCHIP
|
| |
|
| Submission date |
Sep 22, 2015 |
| Last update date |
Sep 23, 2015 |
| Contact name |
Mary-Lee Dequeant |
| E-mail(s) |
[email protected], [email protected], [email protected], [email protected]
|
| Phone |
617-432-6855
|
| Organization name |
Harvard Medical School
|
| Department |
Genetics
|
| Lab |
Norbert Perrimon
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (2) |
| GSE73335 |
Discovery of progenitor signatures by time series synexpression analysis during Drosophila cell immortalization [Microarray Expression] |
| GSE73354 |
Discovery of progenitor signatures by time series synexpression analysis during Drosophila cell immortalization |
|
