|
| Status |
Public on May 25, 2007 |
| Title |
SVGR2 subclone 1-C4 GeneChip U133B |
| Sample type |
RNA |
| |
|
| Source name |
SVGR2 glial cells
|
| Organism |
Homo sapiens |
| Characteristics |
VGR2 cells are glial cells which are derived from SVG-A cells. They were created by subjecting SVG-A cells to multiple rounds of lytic infection by the human polyomavirus JCV. SVGR2 cells are the cells that survived this process and are resistant to JCV infection
|
| Treatment protocol |
Cells were left at 100% confluency before RNA was harvested.
|
| Growth protocol |
Cells were cultured in supplemented with 10% fetal calf serum (Mediatech, Inc.) and maintained in a humidified 37°C CO2 incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed using the Qiagen Rneasy midi kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from SVGR2 glial cells.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
May 16, 2007 |
| Last update date |
May 16, 2007 |
| Contact name |
Kate Manley |
| Organization name |
Brown University
|
| Street address |
70 Ship St.
|
| City |
Providence |
| State/province |
RI |
| ZIP/Postal code |
02903 |
| Country |
USA |
| |
|
| Platform ID |
GPL97 |
| Series (1) |
| GSE7819 |
Comparison of gene expression in SVG-A glial cells and SVGR2 glial cells |
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