|
| Status |
Public on May 05, 2009 |
| Title |
Healthy Twin 28b |
| Sample type |
RNA |
| |
|
| Source name |
Intestinal mucosa biopsy from healthy human individuals
|
| Organism |
Homo sapiens |
| Characteristics |
other: pair 28
gender: male
age: 33
genotype/variation: dizygotic twin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy mini extraction was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using Message Amp II-Biotin (Ambion) and further processed using the standard Affymetrix protocol (Affymetrix manual 2005)
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 µg of cRNA were hybridized for 16 hr at 45C on GeneChip HG U133 2.0 Plus Array (Affymetrix GeneChipHybridization Oven 640). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3037
|
| Description |
Gene expression data from healthy human twins (mucosal biopsies), male, age: 33b
|
| Data processing |
Sample data was processed using GCOS 1.3.0 (Affymetrix default settings) and normalized using GCRMA
|
| |
|
| Submission date |
May 16, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Robert Haesler |
| E-mail(s) |
[email protected]
|
| Phone |
+49 (0) 431 597 5754
|
| Organization name |
University Clinic of Schleswig Holstein
|
| Department |
IKMB / 1st Dept. of Medicine
|
| Lab |
Functional Genomics Platform
|
| Street address |
Schittenhelmstr. 12
|
| City |
Kiel |
| ZIP/Postal code |
24105 |
| Country |
Germany |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE7821 |
Expression data from human intestinal biopsies |
|
| Relations |
| Reanalyzed by |
GSE119087 |
