|
| Status |
Public on May 17, 2007 |
| Title |
No_DFO_control 2 |
| Sample type |
RNA |
| |
|
| Source name |
Untreated U251 cells not stimulated with DFO, biological replicate 3
|
| Organism |
Homo sapiens |
| Characteristics |
cultured U251 cells
refer to ATTC
|
| Treatment protocol |
After the 1-day growth, media was changed and at this time 1 microM of polyamide targeted to the HRE or siRNA against HIF-1a (invitrogen, as per the invitrogen protocol) was added to appropriate wells. Cells were grown for 48 hours more. Echinomycin (100 nM) was added to the appropriate cells, incubataed for 2 hours. DFO was added (300 microM) for 16 hours prior to harvest.
|
| Growth protocol |
U251 cells seeded in 12-well plates in 10% FBS RPMI for 1 day.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DFO were also harvested.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| Scan protocol |
Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| Description |
Untreated LNCaP cells not stimulated with DHT, biological replicate 1
|
| Data processing |
Resolver (Rosetta Biosoftware, Seattle); Affymetrix GeneChip Operating Software (GCOS) integrated into the Rosetta Resolver gene expression data analysis system
|
| |
|
| Submission date |
May 17, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Peter Dervan |
| Organization name |
California Institute of Technology
|
| Street address |
1200 East California Blvd
|
| City |
Pasadena |
| State/province |
CA |
| ZIP/Postal code |
91125 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE7835 |
Modulating hypoxia-inducible transcription by disrupting the HIF-1-DNA interface |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
