|
| Status |
Public on May 20, 2008 |
| Title |
MSC_SA01_2 |
| Sample type |
RNA |
| |
|
| Source name |
Mesenchymal Stem Cells derived from hES cells line SA01, biological replicate 2
|
| Organism |
Homo sapiens |
| Characteristics |
Differentiation of hES cells line SA01 towards MPC-like cells
Mesenchymal stem cells phenotype
|
| Growth protocol |
The hES cell lines were maintained on a feeder layer of mitomycin C-inactivated murine embryonic fibroblast (MEF) cells. The culture media (DMEM/F12 supllemented with 20% KRS and 4ng/ml bFGF) was changed daily and routine passages were performed by mechanical cutting of hES cells on a fresh feeder layer every 4-5 days.
Mesenchymal Stem Cells (MSC) were derived from hES by plating cells on 0,1% Gelatin coated dishes in KO-DMEM medium supplemented with 20% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the cells using the RNeasy Mini kit (Qiagen) according to the manufacturer protocol
|
| Label |
Biotin
|
| Label protocol |
100 ng of total RNA was used to generate biotinylated cRNA using the GeneChip® Two-Cycle Target Labeling and Control Reagents from Affymetrix according to the manufactures protocol
|
| |
|
| Hybridization protocol |
Hybridization, washing and staining procedures of the Affymetrix GeneChip® Human Genome U133 Plus 2.0 were carried out as described in the Affymetrix technical manual (see manufacturer's web site)
|
| Scan protocol |
Scanning procedures of the Affymetrix GeneChip® Human Genome U133 Plus 2.0 were carried out as described in the Affymetrix technical manual (see manufacturer's web site)
|
| Description |
gene expression data for SA01 hES cell line-derived MSC
|
| Data processing |
The statistical treatment of the expression data was achieved using the Array Assist 4.1 software (Stratagene). Normalization was performed using the GC-RMA algorithm.
|
| |
|
| Submission date |
May 23, 2007 |
| Last update date |
May 23, 2007 |
| Contact name |
Christelle ROCHON-BEAUCOURT |
| E-mail(s) |
[email protected]
|
| Fax |
+33169908521
|
| Organization name |
INSERM/UEVE UMR 861/ISTEM,AFM
|
| Street address |
5 rue H. Desbruères
|
| City |
EVRY |
| ZIP/Postal code |
91030 |
| Country |
France |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE7879 |
Comparison of gene expression data between undifferentiated hES cells and MSC-derived hES cells |
|
