|
| Status |
Public on Feb 13, 2017 |
| Title |
Ca9-22_RUNX2 |
| Sample type |
RNA |
| |
|
| Source name |
Ca9-22_RUNX2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Ca9-22
cell type: gingival carcinoma
|
| Treatment protocol |
Cell were selected with blasticidin for two weeks. Stably transduced cells were use for this experiment.
|
| Growth protocol |
Cells were cultured in 5% CO2 incubator at 37℃.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagene RNeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 PLUS 2.0 . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from Ca9-22 cells expressing RUNX2 vector
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Oct 19, 2015 |
| Last update date |
Feb 13, 2017 |
| Contact name |
Wei-Min Chang |
| E-mail(s) |
[email protected]
|
| Phone |
+886227898752
|
| Organization name |
Academia Sinica
|
| Street address |
128 Academia Road, Section 2, Nankang,
|
| City |
Taipei |
| State/province |
Not Applicable |
| ZIP/Postal code |
115 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE74137 |
Expression data from ectopic RUNX2 over-expression in Ca9-22 cells |
|
