|
| Status |
Public on Feb 08, 2017 |
| Title |
ICI182R1a |
| Sample type |
RNA |
| |
|
| Source name |
fulvestrant resistant MCF7 subline ICI182R1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line source: MCF7
cell subline: MCF-7/ICI182R1
cell type: ER+ breast cancer cell line
subline cell type: fulvestrant resistant
|
| Treatment protocol |
FulvR cells were treated with 10^-7M fulvestrant prior to RNA extraction
|
| Growth protocol |
The human breast cancer cell line MCF-7 was originally obtained from The Breast Cancer Task Force Cell Culture Bank, Mason Research Institute (Edinburgh, UK). The MCF-7 cells were gradually adapted to grow in low serum concentration and the fulvestrant-sensitive subline MCF-7/S0.5 was used to establish the four fulvestrant resistant cell lines: MCF-7/ICI164R1 (ICI164R6), MCF-7/ICI182R1 (ICI182R1) and MCF-7/ICI182R6 (ICI182R6) and MCF- by long-term treatment with 10-7Mfulvestrant. Cells were kept within 10 passages throughout the experiments to reduce variability between experimental results.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by lysing cells in Trizol and total RNA was precipitated using 75% ethanol.
|
| Label |
biotin
|
| Label protocol |
dMicroarray Protocol for Affymetrix In Situ Synthesized Oligo Arrays, AfCS Procedure Protocol PP00000174 Microarray Protocol for Affymetrix In Situ Synthesized Oligo Arrays, AfCS Procedure Protocol PP0000017
|
| |
|
| Hybridization protocol |
Microarray Protocol for Affymetrix In Situ Synthesized Oligo Arrays, AfCS Procedure Protocol PP00000174 Microarray Protocol for Affymetrix In Situ Synthesized Oligo Arrays, AfCS Procedure Protocol PP00000175
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 Dx. Standard Affymetrix Cel file scan protocol.
|
| Description |
MCF7 breast cancer cell lines resistant to fulvestrant
|
| Data processing |
Microarray data were normalized and processed by Partek® Genomics Suite using RMA normalization methods
|
| |
|
| Submission date |
Oct 27, 2015 |
| Last update date |
Feb 08, 2017 |
| Contact name |
Daniel Elias |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Southern Denmark
|
| Department |
Cancer and Inflammation Research
|
| Lab |
Ditzel Lab
|
| Street address |
J. B. Winsløwsvej 25
|
| City |
Odense C |
| ZIP/Postal code |
5000 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE74391 |
High CDK6 protects cells from fulvestrant-mediated apoptosis and is a predictor of resistance to fulvestrant in estrogen receptor-positive metastatic breast cancer |
|
