|
| Status |
Public on Nov 03, 2016 |
| Title |
Sample B299: whole liver tissue |
| Sample type |
RNA |
| |
|
| Source name |
whole liver sample
|
| Organism |
Homo sapiens |
| Characteristics |
tissue type: hepatocellular carcinoma
group: HCC
|
| Treatment protocol |
N/A
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from liver tissues using Trizol and following manufacter’s manual instructions (Life Tecnologies, Carlsbad, CA). miRNA quantification was performed using the commercial kit Mir-X miRNA First-Strand Synthesis Kit doing as manufacter’s manual instructions (Takara Bio Europe/Clontech, Saint-Germain-en-Laye, France).
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol using a Gene chip scanner 3000
|
| |
|
| Hybridization protocol |
Hybridization was performed on Affymetrix GeneChip miRNA 2.0 arrays using standard protocols available at www.affymetrix.com.
|
| Scan protocol |
Standard Affymetrix protocol using a Gene chip scanner 3000
|
| Description |
Sample B299: whole liver tissue (HCC group)
|
| Data processing |
Scanned images of microarray chips were analyzed using Expression Console (Affymetrix) with the default settings. Raw data were processed using different algorithms. Detected Above Background algorithm (DABD) and RMA Background Adjustment algorithm were performed in order to remove the background value.
|
| |
|
| Submission date |
Nov 03, 2015 |
| Last update date |
Nov 03, 2016 |
| Contact name |
Juanjo Lozano |
| E-mail(s) |
[email protected]
|
| Organization name |
CIBEREHD
|
| Department |
Plataforma de Bioinformatica
|
| Street address |
C/ Rosselló 153
|
| City |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL14613 |
| Series (1) |
| GSE74618 |
Re-expression of fetal IGF2 as a target for hepatocellular carcinoma therapy |
|
