|
Status |
Public on Nov 13, 2015 |
Title |
HCT116 cells transfected with a ZNF582-AS1 expression vector 1 |
Sample type |
RNA |
|
|
Source name |
HCT116
|
Organism |
Homo sapiens |
Characteristics |
cell line: HCT116 vector: ZNF582-AS1
|
Treatment protocol |
HCT116 cells were transfected with a ZNF582-AS1 expression vector or an empty vector. Seventy-two hours after transfection, total RNA was extracted
|
Growth protocol |
HCT116 cells were cultured in McCoy's 5A Medium supplemented with 10% heat-inactivated fetal bovine serum. The cultures were incubated at 37 ÂșC in a humidified incubator with 5% CO2.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using a RNeasy Mini Kit (QIAGEN).
|
Label |
Cy3
|
Label protocol |
Total RNA (100 ng) was labeled using a Low Input Quick Amp Labeling Kit One-Color (Agilent Technologies) according to manufacturer's instruction.
|
|
|
Hybridization protocol |
Hybridization was performed using a Gene Expression Hybridization kit (Agilent Technologies) according to manufacturer's instruction.
|
Scan protocol |
Array was scanned with an Agilent G2565BA Microarray Scanner.
|
Description |
gene expression in HCT116 cells transfected with a ZNF582-AS1 expression vector (replicate 1)
|
Data processing |
The microarray data were analyzed using GeneSpring GX version 13
|
|
|
Submission date |
Nov 12, 2015 |
Last update date |
Apr 23, 2018 |
Contact name |
Hiromu Suzuki |
E-mail(s) |
[email protected]
|
Phone |
+81-11-611-2111
|
Organization name |
Sapporo Medical University
|
Department |
Department of Molecular Biology
|
Street address |
S1, W17, Chuo-ku
|
City |
Sapporo |
State/province |
Hokkaido |
ZIP/Postal code |
060-8556 |
Country |
Japan |
|
|
Platform ID |
GPL16699 |
Series (1) |
GSE74945 |
A genomic screen for long noncoding RNA genes epigenetically silenced by aberrant DNA methylation in colorectal cancer |
|
Relations |
Reanalyzed by |
GSE113533 |