|
| Status |
Public on Jun 30, 2007 |
| Title |
Suspension cell |
| Sample type |
RNA |
| |
|
| Source name |
Rice suspension cell
|
| Organism |
Oryza sativa |
| Characteristics |
genotype: Japonica Nipponbare
4 weeks suspension cultured cell
|
| Growth protocol |
Rice calli were cultured in N6 liquid medium containing 2,4-D (1 mg/l) in a rotary shaker at 150 rpm at 25 degree for 4 weeks with the medium refreshed every week.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
the suspension cultured cell were collected by centrifuge
total RNA was isolated and purified by Qiagen RNeasy kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degree on GeneChip Rice Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from rice stigma
|
| Data processing |
The data were analyzed with GCOS software (Microarray Suite version 5.0, MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
May 29, 2007 |
| Last update date |
Jul 31, 2009 |
| Contact name |
Wenying Xu |
| E-mail(s) |
[email protected]
|
| Organization name |
Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences
|
| Street address |
Datun Road, Chaoyang District
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE7951 |
Genome-wide gene expression profiling of rice stigma |
|
