|
| Status |
Public on Jun 01, 2016 |
| Title |
Porcinemonocytes_PRRSV&S.suis_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Porcinemonocytes_PRRSV&S.suis
|
| Organism |
Sus scrofa |
| Characteristics |
cell type: Monocytes
infected with: porcine reproductive and respiratory syndrome virus & Streptococcus suis strain P1/7
|
| Treatment protocol |
Cells were plated at 1 x 10^6 cells/ml in 1 ml of complete RPMI and rested 3 h before use. For PRRSV infection, medium was removed and replaced with 300 μl of serum-free complete RPMI with PRRSV IAF-Klop (MOI of 0.1) for 2 h. The wells were washed with PBS twice to remove free virus and 1 ml of complete RPMI was added to each well.For co-infection experiments, cells were infected with PRRSV as described above. After 2 h infection, cells were washed with PBS. Then, S. suis P1/7 was added at a MOI of 0.1 (1 x 105 CFU) for the microarray assay. Bacteria were left in culture with the cells for 12 h. In parallel studies, virus-infected cells were left without bacterial infection (virus infection alone) or were virus-mock infected, similarly incubated and then infected with S. suis (bacterial infection alone).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from porcine monocytes using QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Cyanine 3
|
| Label protocol |
cDNA was labelled with Cyanine 3 Labeling Master Mix according to Manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Standard Agilent hybridization protocol
|
| Scan protocol |
Standard Agilent scanning protocol
|
| Description |
PRRSV-P1_7-T_MGK173-1_US09473739_252644010643_S01_GE1_1100_Jul11_1_2.txt
|
| Data processing |
The data were normalised with VSN method using Flexarray 1.6.2
|
| |
|
| Submission date |
Dec 03, 2015 |
| Last update date |
Jun 01, 2016 |
| Contact name |
Claude Lachance |
| E-mail(s) |
[email protected]
|
| Organization name |
Bio-Rad
|
| Street address |
1329 Meyerside Drive
|
| City |
Mississauga |
| State/province |
Ontario |
| ZIP/Postal code |
L5T 1C9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL16571 |
| Series (1) |
| GSE75687 |
Transcriptional immune response of PRRSV-infected porcine dendritic cells and monocytes to Streptococcus suis infection |
|
