|
| Status |
Public on Aug 01, 2016 |
| Title |
bl_6149 |
| Sample type |
genomic |
| |
|
| Source name |
obese female, peripheral blood mononuclear cells, insulin sensitive
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: peripheral blood mononuclear cells
gender: female
phenotype: obese
disease state: no systemic insulin resistance
insulin resistance: sensitive
individual identifier: 6149
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was prepared from PBMCs using the QIAamp DNA Mini kit (cat no. 51304, Qiagen, Hilden, Germany).
|
| Label |
Biotin
|
| Label protocol |
Standard Illumina protocol for Human Methylation 450K DNA Analysis BeadChip.
|
| |
|
| Hybridization protocol |
Bisulphite-converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation 450K BeadChip using standard Illumina protocol.
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting.
|
| Data processing |
Quantile normalization was applied, followed by BMIQ normalization. Batch effects were checked and removed from the dataset.
Data was normalized and analyzed using GenomeStudio software, R packages and Qlucore software.
|
| |
|
| Submission date |
Dec 22, 2015 |
| Last update date |
Aug 01, 2016 |
| Contact name |
Ingrid Dahlman |
| E-mail(s) |
[email protected]
|
| Organization name |
Karolinska Institute
|
| Department |
Department of Medicine
|
| Street address |
Hälsovägen 7
|
| City |
Huddinge |
| ZIP/Postal code |
14157 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE76285 |
The epigenetic signature of systemic insulin resistance in obese women [BL] |
| GSE76399 |
The epigenetic signature of systemic insulin resistance in obese women |
|
