|
| Status |
Public on Aug 15, 2007 |
| Title |
PAO1 minimal media phosphatidylcholine replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
PAO1 minimal media phosphatidylcholine replicate 1
|
| Organism |
Pseudomonas aeruginosa |
| Characteristics |
Pseudomonas aeruginosa strain PAO1
|
| Growth protocol |
1x M9 minimal media supplemented with 0.4% phosphatidylcholine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Pseudomonas aeruginosa total RNA isolation protocol developed by S. Lory and cleaned up with QIAgen RNease with extra on-column DNaseI treatment
|
| Label |
Biotin
|
| Label protocol |
As per Affymetrix protocol for terminal labeling (used EnzoArray)
|
| |
|
| Hybridization protocol |
As per Affymetrix protocol
|
| Scan protocol |
As per Affymetrix protocol
|
| Description |
Samples processed at Greenwood Biotechnology Facility (Honolulu, HI)
|
| Data processing |
Signal Values determined using MAS 5
|
| |
|
| Submission date |
Jun 05, 2007 |
| Last update date |
Jun 21, 2007 |
| Contact name |
Tung Hoang |
| E-mail(s) |
[email protected]
|
| Phone |
808-956-5035
|
| Organization name |
University of Hawaii at Manoa
|
| Department |
Microbiology
|
| Lab |
LSB 318
|
| Street address |
1800 East-West Road
|
| City |
Honolulu |
| State/province |
HI |
| ZIP/Postal code |
96822 |
| Country |
USA |
| |
|
| Platform ID |
GPL84 |
| Series (1) |
| GSE7704 |
In vivo evidence of Pseudomonas aeruignosa nutrient acquisition and pathogenesis in the cystic fibrosis lung |
|
