|
| Status |
Public on Jun 07, 2007 |
| Title |
RCC099_normal tissue_rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
normal kidney tissue
|
| Organism |
Homo sapiens |
| Characteristics |
birthday 30/11/1930; male; normal tissue mass 12.4g
|
| Treatment protocol |
tissues were shock frozen in liquid nitrogen directly after surgery.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. Incubation, washing, and staining were carried out according to the GeneChip Mapping Assay Manual, using standard protocols on an Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Gene-Chip Scanner 3000.
|
| Description |
HLA-typing: A*02, B*27, B*57
|
| Data processing |
Data were analyzed with the GCOS software (Affymetrix), using default settings for all parameters. Pairwise comparisons were calculated using the respective normal kidney array as baseline. For normalization, 100 housekeeping genes provided by Affymetrix were used (http://www.affymetrix.com/support/technical/mask_files.affx). Relative expression values were calculated from the signal log ratios given by the software.
|
| |
|
| Submission date |
Jun 07, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Andreas Oliver Weinzierl |
| E-mail(s) |
[email protected]
|
| Phone |
004970712987647
|
| Fax |
00497071295653
|
| Organization name |
University of Tuebingen
|
| Department |
Department of Immunology
|
| Lab |
Rammensee
|
| Street address |
Auf der Morgenstelle 15
|
| City |
Tuebingen |
| ZIP/Postal code |
72076 |
| Country |
Germany |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE8050 |
A cryptic VEGF T-cell epitope: Identification and characterization by mass spectrometry and T-cell assays. |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
