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Sample GSM200628 Query DataSets for GSM200628
Status Public on Jun 22, 2007
Title low salt, 7 minutes A
Sample type RNA
 
Source name photosynthetic conditions (10W/m2 white light illumination, no oxygen); low salt stress (0.25% NaCl) during 7 min
Organism Cereibacter sphaeroides 2.4.1
Characteristics strain: 2.4.1
Growth protocol Cells are grown at anoxic photosynthetic (10 W/m2 illumination) conditions; salt stress was induced by the addition of NaCl to the exponentially grown (A600 = 0.4 ± 0.03) cultures to the final concentration of 0.25 %; Cultures were collected at 7 min after addition of NaCl
Extracted molecule total RNA
Extraction protocol RNA isolated with bead beater + RNeasy kits
Label biotin
Label protocol according Affymetrix protocol
 
Hybridization protocol according Affymetrix protocol
Scan protocol according Affymetrix protocol
Description Rhodobacter sphaeroides 2.4.1, Laramie
Data processing RMA (context-dependent) processing for corresponding experimental series was used for data analysis and mentioned in the papers; MAS 5.0 values are depositied here for consistensy with other submissions
 
Submission date Jun 11, 2007
Last update date Aug 14, 2011
Contact name Mark Gomelsky
E-mail(s) [email protected]
Phone 307-766-3522
Organization name University of Wyoming
Department Molecular Biology
Lab Gomelsky
Street address
City Laramie
State/province WY
ZIP/Postal code 82071
Country USA
 
Platform ID GPL162
Series (1)
GSE8082 Rhodobacter sphaeroides salt stress response

Data table header descriptions
ID_REF
MAS5.0_VALUE relative signal of expression, MAS 5.0 in-chip scaling to 500
DETECTION CALL Present if p<0.04 Absent if p>0.06 Marginal if 0.04 < p < 0.06
VALUE RMA values of expression calculated in context of GSE8082 series

Data table
ID_REF MAS5.0_VALUE DETECTION CALL VALUE
1 9.4 A 31
2 3.1 A 30
3 2.4 A 21
4 6.6 A 20
5 1.1 A 21
6 0.8 A 19
7 1.3 A 22
8 1.5 A 21
9 5.8 A 22
10 3.2 A 21
11 2.2 A 19
12 0.5 A 19
13 3.1 A 19
14 0.3 A 19
15 0.3 A 19
16 0.3 A 18
17 3.1 A 19
18 2.7 A 21
19 1.1 A 19
20 0.1 A 18

Total number of rows: 5355

Table truncated, full table size 83 Kbytes.




Supplementary file Size Download File type/resource
GSM200628.CEL.gz 1.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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