|
| Status |
Public on Dec 01, 2008 |
| Title |
ED treated MCF7 wt xenograft, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
MCF7 xenografts
|
| Organism |
Homo sapiens |
| Characteristics |
E2 deprivation (ED) treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was carried out using EZ1 RNA universal tissue kit with BioRobot EZ1 workstation according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 ug of cRNA were hybridized on Human Genome U133 Plus 2.0 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-ws2v5 450.
|
| Scan protocol |
GeneChips were scanned using an Affymetrix GeneChip Scanner (Affymetrix).
|
| Description |
Gene expression data from MCF-7 xenograft tumors that acquired resistance to E2 withdrawal plus tamoxifen.
|
| Data processing |
The data were analyzed with dChip version2006 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to the bottom 10%. of intensity values
|
| |
|
| Submission date |
Jun 15, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Chad Creighton |
| E-mail(s) |
[email protected]
|
| Organization name |
Baylor College of Medicine
|
| Department |
Biostatistics, Ducan Cancer Center
|
| Street address |
One Baylor Plaza, Mail Stop: BCM305
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE8140 |
Expression data from MCF7 wt xenografts |
| GSE8141 |
Expression data from MCF7 wt and MCF7/HER2-18 xenografts |
|
| Relations |
| Reanalyzed by |
GSE119087 |
