|
| Status |
Public on May 31, 2016 |
| Title |
control amygdala, replicate2 (gene-level) |
| Sample type |
RNA |
| |
|
| Source name |
control, brain, amygdala
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: male
age: 8 week-old
agent: control
tissue: brain
tissue region: amygdala
|
| Treatment protocol |
Male mice of 8-week old were subjected to intraperitoneal injection of INTRALIPID or 5 mg/ml DZP at 8:00 and 18:00 for 10 days.
|
| Growth protocol |
Mice were housed in plastic cages containing wood chips and covered with a wire lid. The cages were kept at 24±1 °C under a 12 h light/dark cycle (lights on at 8:00). The diet were standard laboratory food and water ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from each source using RNeasy Lipid Tissue Mini Kit (QIAGEN) according to the manufacturer's protocol. 100 ng of total RNA was subsequently carried out to First-strand cDNA synthesis using GeneChip® WT PLUS Reagent Kit (Affymetrix).
|
| Label |
biotin
|
| Label protocol |
Fragmented single-strand cDNA were subjected to 3' terminal labeling with biotin using GeneChip® WT PLUS Reagent Kit in accordance with manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Fragmented and biotin-labeled single-strand cDNA (5.2 μg each) hybridized on GeneChip® MTA 1.0 for 16 h at 45°C with rotation at 60 rpm using GeneChip® Hybridization Oven 645.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G (Affymetrix).
|
| Description |
CA2
|
| Data processing |
The data were analyzed with Expression Console version 1.4.1 (Affymetrix) using default settings for background correction, normalization and summarization.
affymetrix-algorithm-name = sst-rma-gene-full
affymetrix-algorithm-version = 1.0
|
| |
|
| Submission date |
Jan 11, 2016 |
| Last update date |
May 31, 2016 |
| Contact name |
Shinya Ueno |
| E-mail(s) |
[email protected]
|
| Phone |
+81 172 39 5137
|
| Organization name |
Hirosaki Univ Grad Sch of Med
|
| Department |
Neurophysiology
|
| Street address |
5 Zaifu-cho
|
| City |
Hirosaki |
| State/province |
Aomori Pref. |
| ZIP/Postal code |
036-8562 |
| Country |
Japan |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE76700 |
Whole transcriptome analysis of mouse brain with chronic diazepam treatment |
|
| Relations |
| Reanalyzed by |
GSM2040574 |
