|
| Status |
Public on Jan 31, 2017 |
| Title |
Multiple myeloma. Pair 13. Diagnosis. [gene expression] |
| Sample type |
RNA |
| |
|
| Source name |
MM patient_diagnosis
|
| Organism |
Homo sapiens |
| Characteristics |
subject condition: Multiple myeloma (MM) patient
sample pair id: pair 13
gender: Female
age (yr): 60
patient status: diagnosis
tissue: Bone marrow
cell type: CD138+ plasma cells
|
| Treatment protocol |
Not applicable
|
| Growth protocol |
Not applicable
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tumors were extracted and cells were collected. Total RNA from disgregated cells was extracted using Buffer RLT plus (Qiagen, CA, USA) and purified with All prep DNA/RNA mini Kit (Qiagen, CA, USA).
|
| Label |
biotin
|
| Label protocol |
Labelling and hybridizations were performed according to protocols from Affymetrix. Briefly, 100-300 ng of total RNA were amplified and labeled using the WT Sense Target labelling and control reagents kit (Affymetrix Inc., Santa Clara, CA, USA)
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridized to Human Gene 1.0 ST Array
|
| Scan protocol |
Washing and scanning were performed using GeneChip System of Affymetrix (GeneChip Hybridization Oven 640, GeneChip Fluidics Station 450 and GeneChip Scanner 7G).
|
| Description |
P13D_2152
|
| Data processing |
Expression value for each probe set was calculated using Affymetrix Expression Console that uses RMA (Robust Multi-Array Average) algorithm.
probe group file: HuGene-1_0-st-v1.r4.pgf
meta-probeset file: HuGene-1_0-st-v1.r4.mps
|
| |
|
| Submission date |
Feb 03, 2016 |
| Last update date |
Jan 31, 2017 |
| Contact name |
Norma C. Gutiérrez |
| E-mail(s) |
[email protected]
|
| Phone |
+34923291384
|
| Organization name |
Centro de Investigación del Cáncer de Salamanca
|
| Department |
Hematologia
|
| Lab |
12
|
| Street address |
Campus Miguel de Unamuno
|
| City |
Salamanca |
| State/province |
Salamanca |
| ZIP/Postal code |
37007 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE77539 |
Integrative analysis of DNA copy number, DNA methylation and gene expression in multiple myeloma reveals alterations related to relapse [gene expression] |
| GSE77540 |
Integrative analysis of DNA copy number, DNA methylation and gene expression in multiple myeloma reveals alterations related to relapse |
|
